Journal: Journal of Biological Chemistry

Article Title: Expression Profiles of Arabidopsis thaliana in Mineral Deficiencies Reveal Novel Transporters Involved in Metal Homeostasis

doi: 10.1074/jbc.m309338200

Figure Lengend Snippet: FIG. 6. Functional characterization of AtOPT3. A, hybridization of an 600-bp AtOPT3 cDNA probe, corresponding to the 3-terminal exon, to RNA extracted from Arabidopsis roots of plants grown in control conditions (Ctl), copper (-Cu), manganese (-Mn), zinc (-Zn), and iron (-Fe) deficiency. The ethidium bromide-stained RNA gel is shown for quantification. B, growth of ctr1 expressing AtOPT3 on YPG-Ura plates supplemented with 10 M CuSO4 compared with the mutant transformed with the vector alone. C, growth of smf1 expressing AtOPT3 on manganese-limited medium, with and without 1 mM EGTA compared with the growth of the mutant transformed with the vector alone.

Article Snippet: Genome-wide Analysis Provides Insight into Metal Transport—We have used Affymetrix Arabidopsis DNA chips containing 8,300 genes (which cover about one-third of the ge- FIG. 7.

Techniques: Functional Assay, Hybridization, Control, Staining, Expressing, Mutagenesis, Transformation Assay, Plasmid Preparation

Journal: BMC Plant Biology

Article Title: Light has a specific role in modulating Arabidopsis gene expression at low temperature

doi: 10.1186/1471-2229-8-13

Figure Lengend Snippet: Phosphothreonine-immunoblot of thylakoid proteins isolated from Arabidopsis leaves after four different treatments : Control (Ctr), Cold/Light (C/L), Dark (D) and Cold/Dark (C/D). Below the immunoblot, 77 K fluorescence emission ratios (F732/F685 ± S.D.) of thylakoids from differentially treated plants are given. F732 stands for the fluorescence peak at 732 nm representing the emission from PSI and F685 for the fluorescence peak at 685 nm from PSII. Differences in F732/F685 ratios are related to reversible phosphorylation of the light-harvesting chl a/b proteins (LHCII) and their attachment with PSI (phosphorylated, high ratio) and PSII (non-phosphorylated, low ratio). P-CP43, P-D2, P-D1 denote the phosphorylated proteins of PSII core, P-LHCII denote the LHCII phosphoproteins.

Article Snippet: Arabidopsis cDNA microarray slides are based on the GEM1 clone set (8000 ESTs) purchased from InCyte Genomics, Palo Alto, CA, USA [ ].

Techniques: Western Blot, Isolation, Control, Fluorescence, Phospho-proteomics

Journal: BMC Plant Biology

Article Title: Light has a specific role in modulating Arabidopsis gene expression at low temperature

doi: 10.1186/1471-2229-8-13

Figure Lengend Snippet: Verification of some microarray results using northern blot analysis after four different treatments: Control (Ctr), Cold/Light (C/L), Cold/Dark (C/D) and Dark (D) . Hybridizations were made with genes encoding: four photosystem II light harvesting proteins (LHCB) and the Early Light Inducible Protein (ELIP1); two photosystem I related (PSI) proteins, PSI-N and plastocyanin (PC); two proteins of carbohydrate metabolism, a plastidic fructose bisphoshate aldolase (Pl-FBA) and a pyruvate decarboxylase (PDC1); a ZEP protein involved in zeaxanthin and ABA biosynthesis; four chloroplast targeted proteins involved in oxygen radical scavenging and three cytoplasmic or peroxisomal catalases (CAT); a cold-responsive protein (LTI78/RD29A) and genes encoding a MYB-like (CCA1) and three AP2 transcription factors. The hybridization of the 16S rRNA probe to total RNA is shown in the bottom of the figure.

Article Snippet: Arabidopsis cDNA microarray slides are based on the GEM1 clone set (8000 ESTs) purchased from InCyte Genomics, Palo Alto, CA, USA [ ].

Techniques: Microarray, Northern Blot, Control, Hybridization

Journal: BMC Plant Biology

Article Title: Light has a specific role in modulating Arabidopsis gene expression at low temperature

doi: 10.1186/1471-2229-8-13

Figure Lengend Snippet: Accumulation of oxidative stress related compounds in Arabidopsis leaves after Control, Cold/Light and Cold/Dark treatments . A reddish-brown colour indicates production of oxidized DAB in leaves.

Article Snippet: Arabidopsis cDNA microarray slides are based on the GEM1 clone set (8000 ESTs) purchased from InCyte Genomics, Palo Alto, CA, USA [ ].

Techniques: Control

Journal: BMC Plant Biology

Article Title: Light has a specific role in modulating Arabidopsis gene expression at low temperature

doi: 10.1186/1471-2229-8-13

Figure Lengend Snippet: Western blot analysis of dehydrin proteins (A) and of LHCB and glutathione reductase proteins (B) . Protein samples isolated from Arabidopsis leaves under growth condition (Ctr), after the Cold/Light (C/L) treatment, subsequent recovery for one hour at normal growth conditions (re-1hL), after 8-hour Darkness (D) and after the Cold/Dark (C/D) treatment. A typical result is presented out of three independent western blot experiments.

Article Snippet: Arabidopsis cDNA microarray slides are based on the GEM1 clone set (8000 ESTs) purchased from InCyte Genomics, Palo Alto, CA, USA [ ].

Techniques: Western Blot, Isolation